Hotspot

ndhF-rpl32

SSC · 112665-113672 · 1008 bp

Back to Trachyspermum

Pi 0.00911

MarkerSeek score 64.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1008
variable_sites: 13
pi: 0.00911
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TAAGGCTGCCCAATATCCCT	50.0	50.0	58.0	58.2	1174-1193 bp	1.0	80.7
ndhF-rpl32_p2	TGCCGACGGAAAAAGAAGAA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1172-1191 bp	1.0	80.6
ndhF-rpl32_p3	TTGCCGACGGAAAAAGAAGA	TAAGGCTGCCCAATATCCCT	45.0	50.0	58.0	58.2	1173-1192 bp	1.0	80.6
ndhF-rpl32_p4	GTTGCCGACGGAAAAAGAAG	GACAACGCTTTTAAGGCTGC	50.0	50.0	58.0	58.3	1185-1204 bp	1.0	79.5
ndhF-rpl32_p5	AAAAGAAGAAGTCCCGCTCC	TAAGGCTGCCCAATATCCCT	50.0	50.0	57.8	58.2	1162-1181 bp	1.0	79.5