Hotspot

petN-psbM

LSC · 28909-30045 · 1137 bp

Back to Trachyspermum

Pi 0.00235

MarkerSeek score 60.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1137
variable_sites: 4
pi: 0.00235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1309-1311 bp	1.0	67.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1248-1250 bp	1.0	66.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.2	57.2	1288-1290 bp	1.0	64.9
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1309-1311 bp	1.0	63.7
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	1227-1229 bp	1.0	63.4