Hotspot

trnC-GCA-petN

LSC · 28120-28818 · 699 bp

Back to Trachyspermum

Pi 0.0029

MarkerSeek score 61.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 3
pi: 0.0029
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	785-795 bp	1.0	79.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	745-755 bp	1.0	79.5
trnC-GCA-petN_p3	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	55.0	50.0	57.9	57.6	846-856 bp	1.0	77.9
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	806-816 bp	1.0	77.8
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	763-773 bp	1.0	77.3