Hotspot

rpoB-trnC-GCA

LSC · 26811-28048 · 1238 bp

Back to Trachyspermum

Pi 0.00271

MarkerSeek score 60.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 5
pi: 0.00271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGCCCTGATCCATGAACCTA	CTTACCACTCGGCCATATCG	50.0	55.0	57.8	57.9	1337-1351 bp	1.0	79.0
rpoB-trnC-GCA_p2	AGCCCTGATCCATGAACCTA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1377-1391 bp	1.0	78.9
rpoB-trnC-GCA_p3	GCCCTGATCCATGAACCTAC	CTTACCACTCGGCCATATCG	55.0	55.0	57.7	57.9	1336-1350 bp	1.0	78.5
rpoB-trnC-GCA_p4	GCCCTGATCCATGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.7	58.1	1376-1390 bp	1.0	78.3
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	CTTACCACTCGGCCATATCG	50.0	55.0	58.4	57.9	1352-1366 bp	1.0	77.4