Hotspot

atpH-atpI

LSC · 13304-14433 · 1130 bp

Back to Trachyspermum

Pi 0.00481

MarkerSeek score 63.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 8
pi: 0.00481
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATCGAAGCAAGCCCTACAG	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.0	57.9	1226-1259 bp	1.0	81.3
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1192-1225 bp	1.0	81.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	1248-1281 bp	1.0	80.6
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	1208-1241 bp	1.0	80.4
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	1193-1226 bp	1.0	80.1