Hotspot

ndhG-ndhI

SSC · 124449-124935 · 487 bp

Back to Trachydium

Pi 0.077

MarkerSeek score 68.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 487
variable_sites: 84
pi: 0.077
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCAATCGCACCTATTAACGC	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	1325-1469 bp	1.0	91.4
ndhG-ndhI_p2	GCAATCGCACCTATTAACGC	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	1324-1468 bp	1.0	91.4
ndhG-ndhI_p3	GCAATCGCACCTATTAACGC	TGCAAGGTACATCGGTCAAG	50.0	50.0	58.0	57.9	1309-1453 bp	1.0	91.1
ndhG-ndhI_p4	GCAATCGCACCTATTAACGC	TTCTTTGGGGCGTTTACCAA	50.0	45.0	58.0	57.9	943-1087 bp	0.875	86.3
ndhG-ndhI_p5	GCAATCGCACCTATTAACGC	TTTCTTTGGGGCGTTTACCA	50.0	45.0	58.0	57.9	944-1088 bp	0.875	86.3