Hotspot

rpl32-trnL-UAG

SSC · 119220-119893 · 674 bp

Back to Trachydium

Pi 0.0779
MarkerSeek score 83.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
674
variable_sites
130
pi
0.0779
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TACTGGGCAGCCTTAAAAGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 745-1064 bp 1.0 90.6
rpl32-trnL-UAG_p2 TACTGGGCAGCCTTAAAAGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 746-1065 bp 1.0 90.6
rpl32-trnL-UAG_p3 TACTGGGCAGCCTTAAAAGC GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.2 58.1 1227-1546 bp 1.0 90.0
rpl32-trnL-UAG_p4 TACTGGGCAGCCTTAAAAGC TGAAGTAGCAAAGCCTTGGG 50.0 50.0 58.2 58.1 1228-1547 bp 1.0 90.0
rpl32-trnL-UAG_p5 TACTGGGCAGCCTTAAAAGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 755-1074 bp 1.0 89.7