Hotspot

ndhF-rpl32

SSC · 117904-119054 · 1151 bp

Back to Trachydium

Pi 0.0509

MarkerSeek score 78.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1151
variable_sites: 156
pi: 0.0509
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTGTGAACCAAAACCCTA	TAAGGCTGCCCAGTATCCTT	50.0	50.0	58.0	58.1	1672-1785 bp	0.5	65.3
ndhF-rpl32_p2	CCGTGTGAACCAAAACCCTA	TTAAGGCTGCCCAGTATCCT	50.0	50.0	58.0	58.1	1673-1786 bp	0.5	65.3
ndhF-rpl32_p3	GTTGCCGACGGAAAAAGAAG	AAACTAAAACGGGGCTCCAG	50.0	50.0	58.0	58.1	1591-1649 bp	0.25	53.8
ndhF-rpl32_p4	TGCCGACGGAAAAAGAAGAA	AAACTAAAACGGGGCTCCAG	45.0	50.0	58.0	58.1	1589-1647 bp	0.25	53.7
ndhF-rpl32_p5	CCGTGTGAACCAAAACCCTA	AAACTAAAACGGGGCTCCAG	50.0	50.0	58.0	58.1	1997-2055 bp	0.25	53.6