Hotspot

trnW-CCA-trnP-UGG

LSC · 71492-71638 · 147 bp

Back to Trachydium

Pi 0.0556

MarkerSeek score 79.10

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 147
variable_sites: 26
pi: 0.0556
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	222-331 bp	1.0	90.4
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	214-323 bp	1.0	90.0
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	221-330 bp	1.0	89.9
trnW-CCA-trnP-UGG_p4	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	249-358 bp	1.0	89.4
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	248-357 bp	1.0	89.0