Hotspot

ycf3-trnS-GGA

LSC · 49265-50113 · 849 bp

Back to Trachydium

Pi 0.0362
MarkerSeek score 77.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
849
variable_sites
94
pi
0.0362
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 915-980 bp 1.0 82.8
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 936-1001 bp 1.0 82.2
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 47.6 45.0 58.0 57.8 886-951 bp 1.0 82.1
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 47.6 50.0 58.0 58.3 888-953 bp 1.0 81.6
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 916-981 bp 1.0 80.5