Hotspot

trnE-UUC-trnT-GGU

LSC · 35123-36062 · 940 bp

Back to Trachydium

Pi 0.0502

MarkerSeek score 78.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 133
pi: 0.0502
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	881-1124 bp	0.875	84.4
trnE-UUC-trnT-GGU_p2	TTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.4	57.9	916-1159 bp	0.875	81.0
trnE-UUC-trnT-GGU_p3	CCGTTGCCTCCTTGAAAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.8	57.9	882-1125 bp	0.875	79.0
trnE-UUC-trnT-GGU_p4	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	59.1	57.9	853-1096 bp	0.875	77.2
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	ACCGCTGAGTTAAAAGGGC	50.0	52.6	57.8	58.1	880-1123 bp	0.875	76.8