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petN-psbM

LSC · 33167-33878 · 712 bp

Back to Trachydium

Pi 0.0433

MarkerSeek score 76.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 98
pi: 0.0433
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TGGCTGAGTCTTGAATTCCG	AGTATCCCCTTCTGACTCCG	50.0	55.0	57.9	57.9	1676-2132 bp	0.875	83.0
petN-psbM_p2	GGCTGAGTCTTGAATTCCGT	AGTATCCCCTTCTGACTCCG	50.0	55.0	57.9	57.9	1675-2131 bp	0.875	83.0
petN-psbM_p3	TGGCTGAGTCTTGAATTCCG	TATTCAATTCGCGACGACGA	50.0	45.0	57.9	57.8	1527-1870 bp	0.875	83.0
petN-psbM_p4	AGTCCCTTTTTGACTCTGCG	AGTATCCCCTTCTGACTCCG	50.0	55.0	58.1	57.9	1313-1753 bp	0.875	82.3
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AGTATCCCCTTCTGACTCCG	50.0	55.0	58.2	57.9	1215-1655 bp	0.875	82.1