Hotspot

trnC-GCA-petN

LSC · 32381-33076 · 696 bp

Back to Trachydium

Pi 0.0434

MarkerSeek score 75.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 75
pi: 0.0434
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGATATGGCCGAGTGGTAAG	GGCTTCGTGTCGATCAATCT	55.0	50.0	57.9	58.1	1299-1757 bp	1.0	90.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	GGCTTCGTGTCGATCAATCT	50.0	50.0	58.1	58.1	1259-1717 bp	1.0	90.8
trnC-GCA-petN_p3	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	680-792 bp	1.0	90.3
trnC-GCA-petN_p4	CGATATGGCCGAGTGGTAAG	AACATGTTGCAGAACGGTCT	55.0	45.0	57.9	58.0	894-922 bp	0.75	76.0
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AACATGTTGCAGAACGGTCT	50.0	45.0	58.1	58.0	854-882 bp	0.75	76.0