Hotspot

ycf1

SSC · 127160-132787 · 5628 bp

Back to Trachycarpus

Pi 0.00129

MarkerSeek score 44.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5628
variable_sites: 18
pi: 0.00129
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTAATGTCTGATCCCGCGT	TTGTGCCACTTTTTGATGCA	45.0	40.0	57.3	57.0	290 bp	1.0	67.5
ycf1_p2	AATGTCTGATCCCGCGTAAT	TTGTGCCACTTTTTGATGCA	45.0	40.0	57.1	57.0	287 bp	1.0	65.7
ycf1_p3	ATGTCTGATCCCGCGTAATT	TTGTGCCACTTTTTGATGCA	45.0	40.0	57.1	57.0	286 bp	1.0	65.7
ycf1_p4	TTTAATGTCTGATCCCGCGT	TGTGCCACTTTTTGATGCAT	45.0	40.0	57.3	56.8	289 bp	1.0	65.7
ycf1_p5	TTTAATGTCTGATCCCGCGT	TTTGTGCCACTTTTTGATGCA	45.0	38.1	57.3	57.7	291 bp	1.0	65.3