Hotspot

ndhD

SSC · 118399-119910 · 1512 bp

Back to Trachycarpus

Pi 0.00132

MarkerSeek score 33.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1512
variable_sites: 4
pi: 0.00132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTTATTGGAGTTGGGACCCG	GACAAAAGCCCGTACTCGAT	55.0	50.0	58.0	58.0	2013 bp	1.0	80.0
ndhD_p2	ATGCAGCTCTTTTATGCGGA	GACAAAAGCCCGTACTCGAT	45.0	50.0	58.0	58.0	2322 bp	1.0	80.0
ndhD_p3	TGCAGCTCTTTTATGCGGAT	GACAAAAGCCCGTACTCGAT	45.0	50.0	58.0	58.0	2321 bp	1.0	80.0
ndhD_p4	CTTATTGGAGTTGGGACCCG	CTGCTTCGTCTGGTGTCTAC	55.0	55.0	58.0	58.0	2410 bp	1.0	79.9
ndhD_p5	TGCAGCTCTTTTATGCGGAT	CTGCTTCGTCTGGTGTCTAC	45.0	55.0	58.0	58.0	2718 bp	1.0	79.9