Hotspot

rpl32-trnL-UAG

SSC · 116251-117059 · 809 bp

Back to Trachycarpus

Pi 0.00429
MarkerSeek score 40.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
809
variable_sites
8
pi
0.00429
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTCTACCGGACATTCAA AGCGTGTCTACCGATTTCAC 45.0 50.0 55.4 58.0 896-902 bp 1.0 60.6
rpl32-trnL-UAG_p2 GGTTTCTACCGGACATTCAA CAGCGTGTCTACCGATTTCA 45.0 50.0 55.4 58.0 897-903 bp 1.0 60.5
rpl32-trnL-UAG_p3 GGTTTCTACCGGACATTCAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 55.4 57.9 906-912 bp 1.0 59.6
rpl32-trnL-UAG_p4 GGTTTCTACCGGACATTCAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 55.4 57.8 905-911 bp 1.0 59.0
rpl32-trnL-UAG_p5 GGTTTCTACCGGACATTCAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 55.4 57.8 904-910 bp 1.0 59.0