Hotspot

atpB-rbcL

LSC · 56390-57158 · 769 bp

Back to Trachycarpus

Pi 0.00165

MarkerSeek score 50.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 3
pi: 0.00165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGAAAAACGACATCCAGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	906-907 bp	1.0	78.6
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	884-885 bp	1.0	78.3
atpB-rbcL_p3	GGGGAAAAACGACATCCAGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	58.1	58.4	907-908 bp	1.0	76.2
atpB-rbcL_p4	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	885-886 bp	1.0	75.9
atpB-rbcL_p5	CTGGGCCAATAATTTGAGCG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.5	58.1	885-886 bp	1.0	75.4