Pi
0.00161
MarkerSeek score
48.20
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1042-1053 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1050-1061 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1074-1085 bp | 1.0 | 78.9 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1082-1093 bp | 1.0 | 78.3 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | ATCCAATCAAGTCCGTAGCG | 55.0 | 50.0 | 57.9 | 57.8 | 1065-1076 bp | 1.0 | 78.0 |