Hotspot

trnD-GUC-trnY-GUA

LSC · 31618-32001 · 384 bp

Back to Trachycarpus

Pi 0.00642
MarkerSeek score 35.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
384
variable_sites
5
pi
0.00642
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 530 bp 1.0 77.4
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT GGGGACGGACTGTAAATTCG 50.0 55.0 57.9 58.4 473 bp 1.0 77.3
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA GGGACGGACTGTAAATTCGT 55.0 50.0 57.9 57.6 529 bp 1.0 77.2
trnD-GUC-trnY-GUA_p4 GTGCTCTGACCGATTGAACT GGGACGGACTGTAAATTCGT 50.0 50.0 57.9 57.6 472 bp 1.0 77.2
trnD-GUC-trnY-GUA_p5 ATCGGATCCTAGTTCGGGAC GGGGACGGACTGTAAATTCG 55.0 55.0 58.4 58.4 533 bp 1.0 74.9