Hotspot

rpoB

LSC · 23778-26987 · 3210 bp

Back to Trachelospermum

Pi 0.00249

MarkerSeek score 43.20

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3210
variable_sites: 8
pi: 0.00249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GCTCGCTAATGAGTACTGCA	ACCGATACCGACAAGATCCT	50.0	50.0	57.8	57.9	259 bp	1.0	78.4
rpoB_p2	ACGCTCGCTAATGAGTACTG	ACCGATACCGACAAGATCCT	50.0	50.0	57.5	57.9	261 bp	1.0	76.2
rpoB_p3	ACCAAACGCTCGCTAATGAG	ACCGATACCGACAAGATCCT	50.0	50.0	58.6	57.9	266 bp	1.0	74.9
rpoB_p4	CCAAACGCTCGCTAATGAGT	ACCGATACCGACAAGATCCT	50.0	50.0	58.6	57.9	265 bp	1.0	74.9
rpoB_p5	TACCAAACGCTCGCTAATGA	ACCGATACCGACAAGATCCT	45.0	50.0	57.3	57.9	267 bp	1.0	74.8