Hotspot

ndhF

SSC · 111501-113729 · 2229 bp

Back to Trachelospermum

Pi 0.00496

MarkerSeek score 60.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 11
pi: 0.00496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCCCCGTTTATGAGGATTC	GAGGTAAGATCCGGTCAATCG	50.0	52.4	57.4	58.0	2376-2410 bp	1.0	68.3
ndhF_p2	GCCCCGTTTATGAGGATTCT	GAGGTAAGATCCGGTCAATCG	50.0	52.4	57.4	58.0	2375-2409 bp	1.0	68.3
ndhF_p3	AGCCCCGTTTATGAGGATTC	AGGTAAGATCCGGTCAATCG	50.0	50.0	57.4	56.8	2375-2409 bp	1.0	66.9
ndhF_p4	GCCCCGTTTATGAGGATTCT	AGGTAAGATCCGGTCAATCG	50.0	50.0	57.4	56.8	2374-2408 bp	1.0	66.9
ndhF_p5	GCCCCGTTTATGAGGATTCTT	GAGGTAAGATCCGGTCAATCG	47.6	52.4	58.1	58.0	2375-2409 bp	1.0	64.8