Hotspot

matK

LSC · 1958-3475 · 1518 bp

Back to Trachelospermum

Pi 0.00527

MarkerSeek score 61.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1518
variable_sites: 8
pi: 0.00527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACAAGCTACTTACATGCTACATCA	GCTAACCCCAAAATCGCCTA	37.5	50.0	58.0	58.0	1674 bp	1.0	47.8
matK_p2	AGCTACTTACATGCTACATCAACA	GCTAACCCCAAAATCGCCTA	37.5	50.0	58.0	58.0	1671 bp	1.0	47.8
matK_p3	GCTACTTACATGCTACATCAACA	GCTAACCCCAAAATCGCCTA	39.1	50.0	56.7	58.0	1670 bp	1.0	45.6
matK_p4	ACAAGCTACTTACATGCTACA	GCTAACCCCAAAATCGCCTA	38.1	50.0	54.7	58.0	1674 bp	1.0	45.5
matK_p5	AGCTACTTACATGCTACATCAACA	TGTTTTGACTCTATCGCGCT	37.5	45.0	58.0	57.6	1702 bp	1.0	45.3