Hotspot

rps14-psaB

LSC · 38396-38528 · 133 bp

Back to Trachelospermum

Pi 0.015

MarkerSeek score 61.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 2
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CTCCCGCTGAATCAAACCTT	CCTGATTGCCTCTACATCGG	50.0	55.0	58.2	57.8	197 bp	1.0	78.1
rps14-psaB_p2	CTCCCGCTGAATCAAACCTT	CTGATTGCCTCTACATCGGG	50.0	55.0	58.2	57.8	196 bp	1.0	78.1
rps14-psaB_p3	CTCCCGCTGAATCAAACCTT	CACTTATGCGGCTTTCCTGA	50.0	50.0	58.2	58.3	212 bp	1.0	77.3
rps14-psaB_p4	CTCCCGCTGAATCAAACCTT	TCACTTATGCGGCTTTCCTG	50.0	50.0	58.2	58.3	213 bp	1.0	77.3
rps14-psaB_p5	TCCCGCTGAATCAAACCTTT	CTGATTGCCTCTACATCGGG	45.0	55.0	57.7	57.8	195 bp	1.0	77.2