Hotspot

psbC-trnS-UGA

LSC · 36487-36743 · 257 bp

Back to Trachelospermum

Pi 0.0195
MarkerSeek score 63.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
257
variable_sites
5
pi
0.0195
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 379-383 bp 1.0 79.6
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 387-391 bp 1.0 79.2
psbC-trnS-UGA_p3 TCGTAGGTCATTTGTGGCAC GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.2 58.0 459-463 bp 1.0 79.1
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC ATAGCCCCGGTCTTGAAAAC 55.0 50.0 58.2 57.9 359-363 bp 1.0 78.8
psbC-trnS-UGA_p5 TCGTAGGTCATTTGTGGCAC GTCTTTCGGAGAGATGGCTG 50.0 55.0 58.2 58.1 467-471 bp 1.0 78.7