Hotspot

trnE-UUC-trnT-GGU

LSC · 31812-32726 · 915 bp

Back to Trachelospermum

Pi 0.00344

MarkerSeek score 56.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 915
variable_sites: 3
pi: 0.00344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGAGATGTCCTGGACCAC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	970-1004 bp	1.0	82.6
trnE-UUC-trnT-GGU_p2	GAGAGATGTCCTGGACCACT	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	969-1003 bp	1.0	82.6
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	985-1019 bp	1.0	82.3
trnE-UUC-trnT-GGU_p4	GTCCTGGACCACTAGACGAT	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	962-996 bp	1.0	81.8
trnE-UUC-trnT-GGU_p5	TCCTGGACCACTAGACGATG	GAACCGATGACTTACGCCTT	55.0	50.0	58.2	58.0	961-995 bp	1.0	81.8