Hotspot

trnC-GCA-petN

LSC · 28297-29171 · 875 bp

Back to Trachelospermum

Pi 0.0114

MarkerSeek score 58.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 10
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	CACTACGAGCGAAAGGGAAA	50.0	50.0	58.1	57.9	973 bp	1.0	79.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	ACGAGCGAAAGGGAAAATGT	50.0	45.0	58.1	58.1	969 bp	1.0	79.3
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	931 bp	1.0	79.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCCTAGAGTCCGCTTCTTCC	50.0	55.0	58.1	58.2	996 bp	1.0	78.3
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CACTACGAGCGAAAGGGAAA	50.0	50.0	58.4	57.9	991 bp	1.0	77.4