Hotspot

rpoB-trnC-GCA

LSC · 26988-28225 · 1238 bp

Back to Trachelospermum

Pi 0.0235

MarkerSeek score 63.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 29
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCCCCCGATCGTTTTGAAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1327-1362 bp	1.0	80.9
rpoB-trnC-GCA_p2	CTCCCCCGATCGTTTTGAAT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1309-1344 bp	1.0	78.7
rpoB-trnC-GCA_p3	CGCCAAACCCTGATCAAGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1402-1437 bp	1.0	77.4
rpoB-trnC-GCA_p4	TCCCCCGATCGTTTTGAATT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.5	58.1	1326-1361 bp	1.0	77.2
rpoB-trnC-GCA_p5	CTCCCCCGATCGTTTTGAAT	TTTGTTGATCAGGCGACACC	50.0	50.0	58.0	58.8	1346-1381 bp	1.0	75.4