Hotspot

atpH-atpI

LSC · 13549-14747 · 1199 bp

Back to Trachelospermum

Pi 0.00348

MarkerSeek score 64.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1199
variable_sites: 4
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	1236-1286 bp	1.0	82.1
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CCGCAGCTTATATCGGTGAA	45.0	50.0	58.0	57.9	1222-1272 bp	1.0	82.0
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.1	57.9	1319-1369 bp	1.0	81.0
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	57.9	57.9	1323-1373 bp	1.0	81.0
atpH-atpI_p5	TACCCTCTACAGCTTGACCC	CCGCAGCTTATATCGGTGAA	55.0	50.0	58.1	57.9	1305-1355 bp	1.0	81.0