Hotspot

ycf1

SSC · 128412-134063 · 5652 bp

Back to Toxicodendron

Pi 0.00755

MarkerSeek score 45.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5652
variable_sites: 97
pi: 0.00755
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTATTCGCTCGATTTCGCCA	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	58.1	58.0	1145 bp	1.0	81.2
ycf1_p2	CGCTCGATTTCGCCATTTTT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	58.1	58.0	1140 bp	1.0	80.8
ycf1_p3	CCGTAATGGGTCTGCTTCAA	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	57.9	58.0	1257 bp	1.0	80.8
ycf1_p4	TATTCGCTCGATTTCGCCAT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.9	58.0	1144 bp	1.0	80.5
ycf1_p5	CTCCTTAACCAGGTTTGCCA	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	57.7	58.0	1070 bp	0.6	62.5