Hotspot

petD

LSC · 80561-81085 · 525 bp

Back to Toxicodendron

Pi 0.00495

MarkerSeek score 43.90

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 525
variable_sites: 6
pi: 0.00495
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GTATCCCCTTGGTTGGGTTG	ACTTTGAAGCGACTACTCCC	55.0	50.0	58.2	57.3	644 bp	1.0	73.6
petD_p2	TATCCCCTTGGTTGGGTTGA	ACTTTGAAGCGACTACTCCC	50.0	50.0	58.2	57.3	643 bp	1.0	72.9
petD_p3	GTTGATTCTTGCTTGAGCCG	ACTTTGAAGCGACTACTCCC	50.0	50.0	57.7	57.3	628 bp	1.0	72.8
petD_p4	ATGTATCCCCTTGGTTGGGT	ACTTTGAAGCGACTACTCCC	50.0	50.0	58.3	57.3	646 bp	1.0	72.3
petD_p5	CCCTTGGTTGGGTTGATTCT	ACTTTGAAGCGACTACTCCC	50.0	50.0	57.7	57.3	639 bp	1.0	72.3