Hotspot

ndhF-rpl32

SSC · 116383-117434 · 1052 bp

Back to Toxicodendron

Pi 0.0117

MarkerSeek score 58.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1052
variable_sites: 27
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTTTTTGACAAGCACTGGCC	CGTGGAAAGGGATTTCGCTA	50.0	50.0	58.1	58.0	1573-1714 bp	1.0	84.3
ndhF-rpl32_p2	CTGGAAGTGGAACGAAAGGT	CGTGGAAAGGGATTTCGCTA	50.0	50.0	57.7	58.0	1093-1234 bp	1.0	83.8
ndhF-rpl32_p3	CTTTTTGACAAGCACTGGCC	TCGAATCAGGCTGAGTCAGA	50.0	50.0	58.1	58.2	1647-1788 bp	1.0	83.1
ndhF-rpl32_p4	CTTTTTGACAAGCACTGGCC	AAAAGAAGGACCAAACCCCC	50.0	50.0	58.1	57.9	1946-1955 bp	0.4	55.1
ndhF-rpl32_p5	CTTTTTGACAAGCACTGGCC	GAAGGACCAAACCCCCTTTT	50.0	50.0	58.1	57.9	1942-1951 bp	0.4	55.1