Hotspot

petD-rpoA

LSC · 81086-81342 · 257 bp

Back to Toxicodendron

Pi 0.0416

MarkerSeek score 67.00

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 19
pi: 0.0416
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TAATGGTTTCAGTACCCGCG	ATATATGGCACTCGTGACGC	50.0	50.0	58.0	57.9	1125-1132 bp	1.0	82.2
petD-rpoA_p2	AGCTGTTGGGCGTTCTTTTA	ATATATGGCACTCGTGACGC	45.0	50.0	58.0	57.9	1143-1150 bp	1.0	82.1
petD-rpoA_p3	TAATGGTTTCAGTACCCGCG	GTGTCAAAGGTCCGGGATAC	50.0	55.0	58.0	58.0	1104 bp	0.2	47.7
petD-rpoA_p4	AGCTGTTGGGCGTTCTTTTA	GTGTCAAAGGTCCGGGATAC	45.0	55.0	58.0	58.0	1122 bp	0.2	47.6
petD-rpoA_p5	CAAAATCCATTTCGCCGTCC	GTGTCAAAGGTCCGGGATAC	50.0	55.0	58.1	58.0	1042 bp	0.2	47.1