Hotspot

trnW-CCA-trnP-UGG

LSC · 69674-69845 · 172 bp

Back to Toxicodendron

Pi 0.0355
MarkerSeek score 73.00
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
172
variable_sites
14
pi
0.0355
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 237-242 bp 1.0 84.2
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 239-244 bp 1.0 84.1
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 240-245 bp 1.0 84.1
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 250-255 bp 1.0 84.0
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 251-256 bp 1.0 84.0