Hotspot

trnE-UUC-trnT-GGU

LSC · 33198-34027 · 830 bp

Back to Toxicodendron

Pi 0.00723

MarkerSeek score 65.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 13
pi: 0.00723
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	963-977 bp	1.0	80.7
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	926-940 bp	1.0	80.6
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	942-956 bp	1.0	80.2
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	905-919 bp	1.0	80.2
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	939-953 bp	1.0	80.1