Hotspot

trnC-GCA-petN

LSC · 29674-30351 · 678 bp

Back to Toxicodendron

Pi 0.00895

MarkerSeek score 63.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 13
pi: 0.00895
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AACAAAACAAGCAAACGGGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.0	58.2	637-824 bp	1.0	84.0
trnC-GCA-petN_p2	AACAAAACAAGCAAACGGGG	AGAGTCCACTTCTTCCCCAT	45.0	50.0	58.0	57.6	698-885 bp	1.0	82.3
trnC-GCA-petN_p3	CAAAACAAGCAAACGGGGAA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.7	58.2	635-822 bp	1.0	81.7
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	565-752 bp	1.0	81.4
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	540-727 bp	1.0	80.5