Hotspot

atpA-atpF

LSC · 13143-13198 · 56 bp

Back to Toxicodendron

Pi 0.0232

MarkerSeek score 64.30

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 3
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TGGGGCGCTGAAAGAAATAA	50.0	45.0	58.0	57.8	198-200 bp	1.0	80.4
atpA-atpF_p2	ATGCCATCGCCTACTTGAAG	TTGGGGCGCTGAAAGAAATA	50.0	45.0	58.0	57.8	199-201 bp	1.0	80.4
atpA-atpF_p3	GATGCCATCGCCTACTTGAA	TGGGGCGCTGAAAGAAATAA	50.0	45.0	58.0	57.8	199-201 bp	1.0	80.4
atpA-atpF_p4	GATGCCATCGCCTACTTGAA	TTGGGGCGCTGAAAGAAATA	50.0	45.0	58.0	57.8	200-202 bp	1.0	80.3
atpA-atpF_p5	ATGCCATCGCCTACTTGAAG	GGGGCGCTGAAAGAAATAAC	50.0	50.0	58.0	57.4	197-199 bp	1.0	77.6