Hotspot

ndhE

SSC · 119509-119814 · 306 bp

Back to Tournefortia

Pi 0.0196

MarkerSeek score 49.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 6
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GACAGGCGGATTCACATCTT	TTCATCTTTGGACCGTTGGG	50.0	50.0	58.0	58.1	1196-1209 bp	1.0	82.9
ndhE_p2	GACAGGCGGATTCACATCTT	TCTTTCTGGGATCGGGTCTT	50.0	50.0	58.0	58.0	1427-1440 bp	1.0	82.8
ndhE_p3	GACCCATACTGCGAGTTGTT	TCTTTCTGGGATCGGGTCTT	50.0	50.0	57.9	58.0	1487-1500 bp	1.0	82.2
ndhE_p4	CCCGTGCTCGAAAATCAAAC	TCTTTCTGGGATCGGGTCTT	50.0	50.0	58.0	58.0	1598 bp	0.5	59.3
ndhE_p5	CCCGTGCTCGAAAATCAAAC	TTCATCTTTGGACCGTTGGG	50.0	50.0	58.0	58.1	1367 bp	0.5	58.9