Hotspot

rps15-ycf1

SSC · 125245-125612 · 368 bp

Back to Tournefortia

Pi 0.0468

MarkerSeek score 76.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 368
variable_sites: 16
pi: 0.0468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGACCCTCTGTTTTCTTCTTGAG	TCCACGATTGAAAATTCGTT	43.5	35.0	58.0	53.8	419-444 bp	1.0	47.0
rps15-ycf1_p2	GACCCTCTGTTTTCTTCTTGAGA	TCCACGATTGAAAATTCGTT	43.5	35.0	58.0	53.8	418-443 bp	1.0	47.0
rps15-ycf1_p3	ACCCTCTGTTTTCTTCTTGAGA	TCCACGATTGAAAATTCGTT	40.9	35.0	57.0	53.8	417-442 bp	1.0	47.0
rps15-ycf1_p4	AGACCCTCTGTTTTCTTCTTGA	TCCACGATTGAAAATTCGTT	40.9	35.0	57.0	53.8	419-444 bp	1.0	47.0
rps15-ycf1_p5	ACTTGAAATTCAACAGACCCTCT	TCCACGATTGAAAATTCGTT	39.1	35.0	57.9	53.8	433-458 bp	1.0	46.9