Hotspot

ndhE-ndhG

SSC · 119815-120032 · 218 bp

Back to Tournefortia

Pi 0.144

MarkerSeek score 80.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 218
variable_sites: 29
pi: 0.144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GGCACTCAAAACAAGTACATGT	AGGTGCAATTGCTGTAGCTC	40.9	50.0	57.4	58.5	266-279 bp	1.0	67.1
ndhE-ndhG_p2	AGGCACTCAAAACAAGTACATG	AGGTGCAATTGCTGTAGCTC	40.9	50.0	57.2	58.5	267-280 bp	1.0	65.0
ndhE-ndhG_p3	AGGCACTCAAAACAAGTACA	AGGTGCAATTGCTGTAGCTC	40.0	50.0	55.1	58.5	267-280 bp	1.0	64.6
ndhE-ndhG_p4	GGCACTCAAAACAAGTACATGT	GCAATTGCTGTAGCTCGTCA	40.9	50.0	57.4	58.9	262-275 bp	1.0	64.1
ndhE-ndhG_p5	GGCACTCAAAACAAGTACATGT	TGCAATTGCTGTAGCTCGTC	40.9	50.0	57.4	58.9	263-276 bp	1.0	64.1