Hotspot

rpl32-trnL-UAG

SSC · 115245-115984 · 740 bp

Back to Tournefortia

Pi 0.0442
MarkerSeek score 77.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
740
variable_sites
31
pi
0.0442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGTTTTGTTGTGCGACAAACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 57.7 57.9 778-809 bp 1.0 75.6
rpl32-trnL-UAG_p2 AGTTTTGTTGTGCGACAAACA AGCAGCGTGTCTACCAATTT 38.1 45.0 57.7 57.8 771-802 bp 1.0 75.2
rpl32-trnL-UAG_p3 AGTTTTGTTGTGCGACAAACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 57.7 57.8 777-808 bp 1.0 75.1
rpl32-trnL-UAG_p4 AGTTTTGTTGTGCGACAAACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 57.7 57.8 776-807 bp 1.0 75.1
rpl32-trnL-UAG_p5 AGTTTTGTTGTGCGACAAACA GAGCAGCGTGTCTACCAATT 38.1 50.0 57.7 58.3 772-803 bp 1.0 74.5