Hotspot

petA-psbJ

LSC · 64798-65828 · 1031 bp

Back to Tournefortia

Pi 0.0434

MarkerSeek score 70.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 44
pi: 0.0434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCATCAGATGGACGTCAAG	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1871-1882 bp	1.0	82.7
petA-psbJ_p2	TGCATCAGATGGACGTCAAG	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1870-1881 bp	1.0	82.7
petA-psbJ_p3	GCATCAGATGGACGTCAAGT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1869-1880 bp	1.0	82.7
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2153-2164 bp	1.0	82.6
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2152-2163 bp	1.0	82.6