Hotspot

accD-psaI

LSC · 60441-61179 · 739 bp

Back to Tournefortia

Pi 0.0405

MarkerSeek score 70.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 29
pi: 0.0405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	912-999 bp	1.0	84.0
accD-psaI_p2	GCCTACATTGCATTTGCTGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1001-1088 bp	1.0	83.4
accD-psaI_p3	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	918-1005 bp	1.0	83.4
accD-psaI_p4	CGGGAATAGGCCGACTAAAC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1342-1429 bp	1.0	82.2
accD-psaI_p5	GGGAATAGGCCGACTAAACG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1341-1428 bp	1.0	82.2