Hotspot

trnF-GAA-ndhJ

LSC · 49556-50220 · 665 bp

Back to Tournefortia

Pi 0.0392

MarkerSeek score 80.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 26
pi: 0.0392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TAGGATGGCCCCTACGTAAG	55.0	55.0	58.0	58.0	800-820 bp	1.0	82.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TAGGATGGCCCCTACGTAAG	50.0	55.0	58.1	58.0	764-784 bp	1.0	82.3
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	TAGGATGGCCCCTACGTAAG	55.0	55.0	57.9	58.0	788-808 bp	1.0	81.6
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	842-862 bp	1.0	81.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GATAGGATGGCCCCTACGTA	55.0	55.0	58.0	57.8	802-822 bp	1.0	80.8