Hotspot

trnE-UUC-trnT-GGU

LSC · 32256-32695 · 440 bp

Back to Tournefortia

Pi 0.0488

MarkerSeek score 74.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 20
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CCTGAACCACTAGACGATGG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	497-519 bp	1.0	82.4
trnE-UUC-trnT-GGU_p2	CTGAACCACTAGACGATGGG	GAACCGATGACTTACGCCTT	55.0	50.0	57.4	58.0	496-518 bp	1.0	82.4
trnE-UUC-trnT-GGU_p3	CCTGAACCACTAGACGATGG	CCATGGCGTTACTCTACCAC	55.0	55.0	57.4	58.1	476-498 bp	1.0	82.1
trnE-UUC-trnT-GGU_p4	CTGAACCACTAGACGATGGG	CCATGGCGTTACTCTACCAC	55.0	55.0	57.4	58.1	475-497 bp	1.0	82.1
trnE-UUC-trnT-GGU_p5	CTGAACCACTAGACGATGGG	TGGCGTTACTCTACCACTGA	55.0	50.0	57.4	58.1	472-494 bp	1.0	81.9