Hotspot

ycf2

Genome · 87706-94494 · 6789 bp

Back to Torreya

Pi 0.00622

MarkerSeek score 44.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6789
variable_sites: 88
pi: 0.00622
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCAGAGAACCCAGGACCAAT	AGCATCTTGTCGCCTTTTCA	50.0	45.0	58.0	58.1	804-939 bp	1.0	84.2
ycf2_p2	TCAGAGAACCCAGGACCAAT	TGAAAAGCATCTTGTCGCCT	50.0	45.0	58.0	58.1	809-944 bp	1.0	84.1
ycf2_p3	TCAGAGAACCCAGGACCAAT	TCCATCCGGGATCTGATGAA	50.0	50.0	58.0	57.9	916-1051 bp	1.0	83.6
ycf2_p4	ATCTCAGAGAACCCAGGACC	AGCATCTTGTCGCCTTTTCA	55.0	45.0	58.1	58.1	807-942 bp	1.0	83.5
ycf2_p5	ATCTCAGAGAACCCAGGACC	TCCATCCGGGATCTGATGAA	55.0	50.0	58.1	57.9	919-1054 bp	1.0	82.9