Hotspot

rps18

Genome · 63138-63629 · 492 bp

Back to Torreya

Pi 0.00528

MarkerSeek score 60.80

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 492
variable_sites: 5
pi: 0.00528
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	ATCCGATTCGATCCAGGAGT	AACAAAATCTGTCACTGTCA	50.0	35.0	58.0	53.0	559-589 bp	1.0	44.7
rps18_p2	ATCCGATTCGATCCAGGAGT	ACAAAATCTGTCACTGTCAA	50.0	35.0	58.0	53.0	558-588 bp	1.0	44.7
rps18_p3	ATCCGATTCGATCCAGGAGT	AACAAAATCTGTCACTGTCAA	50.0	33.3	58.0	53.9	559-589 bp	1.0	44.7
rps18_p4	AGATCCGATTCGATCCAGGA	AACAAAATCTGTCACTGTCA	50.0	35.0	57.7	53.0	561-591 bp	1.0	44.7
rps18_p5	AGATCCGATTCGATCCAGGA	ACAAAATCTGTCACTGTCAA	50.0	35.0	57.7	53.0	560-590 bp	1.0	44.7