Hotspot

chlL-trnH-GUG

Genome · 136053-136479 · 427 bp

Back to Torreya

Pi 0.0136

MarkerSeek score 59.00

Primer available yes

Label chlL-trnH-GUG

Feature Metadata

label_name: chlL-trnH-GUG
feature_type: igs
region: Genome
length_bp: 427
variable_sites: 13
pi: 0.0136
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnH-GUG_p1	ACCAATTCCGCCTTTTCCAT	GACGTAGCCAAGTGGTTCAA	45.0	50.0	58.1	58.1	535-548 bp	1.0	80.4
chlL-trnH-GUG_p2	GTCTGGCTAGGGCAATTGAA	GACGTAGCCAAGTGGTTCAA	50.0	50.0	57.9	58.1	578-591 bp	1.0	79.8
chlL-trnH-GUG_p3	TTTTCACCACGTCTGGCTAG	GACGTAGCCAAGTGGTTCAA	50.0	50.0	57.8	58.1	588-601 bp	1.0	79.4
chlL-trnH-GUG_p4	ACCAATTCCGCCTTTTCCAT	ATTATTTGGGGCGGACGTAG	45.0	50.0	58.1	57.8	548-561 bp	1.0	79.4
chlL-trnH-GUG_p5	ACCAATTCCGCCTTTTCCAT	CTATTATTTGGGGCGGACGT	45.0	50.0	58.1	57.8	550-563 bp	1.0	79.4