Hotspot

ycf1-trnN-GUU

Genome · 132434-133356 · 923 bp

Back to Torreya

Pi 0.045

MarkerSeek score 79.60

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: Genome
length_bp: 923
variable_sites: 79
pi: 0.045
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	CATCCGGCGACAGTACTTAG	GAAGACTTGGATCGGACCAC	55.0	55.0	57.9	58.0	1822-1961 bp	1.0	87.2
ycf1-trnN-GUU_p2	CATCCGGCGACAGTACTTAG	AATTGGTTACCCGACCCCTA	55.0	50.0	57.9	58.0	1860-1999 bp	1.0	87.0
ycf1-trnN-GUU_p3	CATCCGGCGACAGTACTTAG	ACTTGGATCGGACCACCTTA	55.0	50.0	57.9	58.1	1818-1957 bp	1.0	86.9
ycf1-trnN-GUU_p4	CATCCGGCGACAGTACTTAG	TTGGATCGGACCACCTTAGT	55.0	50.0	57.9	58.1	1816-1955 bp	1.0	86.9
ycf1-trnN-GUU_p5	GGGGTTCTGCCATAACACAT	GAAGACTTGGATCGGACCAC	50.0	55.0	57.9	58.0	1984-2123 bp	1.0	86.8