Hotspot

trnR-ACG-trnN-GUU

Genome · 109973-110513 · 541 bp

Back to Torreya

Pi 0.0177
MarkerSeek score 49.00
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
Genome
length_bp
541
variable_sites
17
pi
0.0177
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG GTCGTAGGTTCAAATCCTATTTGA 55.0 37.5 57.8 56.8 631-688 bp 1.0 42.1
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG GTCGTAGGTTCAAATCCTATTTGAG 55.0 40.0 57.8 57.8 631-688 bp 1.0 42.1
trnR-ACG-trnN-GUU_p3 GAACAGAACAGATCCCGTGG GTCGTAGGTTCAAATCCTATTTGA 55.0 37.5 58.3 56.8 668-725 bp 1.0 42.0
trnR-ACG-trnN-GUU_p4 GGAACAGAACAGATCCCGTG GTCGTAGGTTCAAATCCTATTTGA 55.0 37.5 58.3 56.8 669-726 bp 1.0 42.0
trnR-ACG-trnN-GUU_p5 GAACAGAACAGATCCCGTGG GTCGTAGGTTCAAATCCTATTTGAG 55.0 40.0 58.3 57.8 668-725 bp 1.0 42.0