Hotspot

ycf2-trnL-CAA

Genome · 94495-95164 · 670 bp

Back to Torreya

Pi 0.012

MarkerSeek score 55.00

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: Genome
length_bp: 670
variable_sites: 16
pi: 0.012
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	CGGAAGGATTGAATAAGAATAACA	AAATGGTAGACACGCGAGAC	33.3	50.0	55.0	58.0	769-779 bp	1.0	42.0
ycf2-trnL-CAA_p2	CGGAAGGATTGAATAAGAATAACA	TGAAATGGTAGACACGCGAG	33.3	50.0	55.0	58.0	771-781 bp	1.0	42.0
ycf2-trnL-CAA_p3	CGGAAGGATTGAATAAGAATAACA	AATCTCGTGCTAAACAGCGT	33.3	45.0	55.0	57.9	745-755 bp	1.0	42.0
ycf2-trnL-CAA_p4	CGGAAGGATTGAATAAGAATAACA	TGGTGAAATGGTAGACACGC	33.3	50.0	55.0	58.2	774-784 bp	1.0	42.0
ycf2-trnL-CAA_p5	CGGAAGGATTGAATAAGAATAACA	GGGATGCCTTGATGGTGAAA	33.3	50.0	55.0	58.1	786-796 bp	1.0	41.9